Journal
/
Biology
/
In vivo Imaging of Intact Drosophila Larvae at Sub-cellular Resolution
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal Biology
In vivo Imaging of Intact Drosophila Larvae at Sub-cellular Resolution

In vivo Imaging of Intact Drosophila Larvae at Sub-cellular Resolution

DOI:
10.3791/2249-v

•

17:51 min

•

September 10, 2010

•

, , , , ,
1Junior Research Group Synaptic Plasticity, Hertie Institute for Clinical Brain Research,University of Tübingen, 2Graduate School of Cellular and Molecular Neuroscience,University of Tübingen

Chapters

  • 00:00Title
  • 00:05Introduction
  • 02:01Preparing Larva
  • 03:00Assembly of the Imaging Chamber
  • 04:36Anesthetization of the Larva
  • 07:48Imaging
  • 14:39Recovery from Anesthetization

Summary

Automatic Translation

Automatic Translation

This protocol describes a reliable method for anesthetization and imaging of intact Drosophila melanogaster larvae. We have utilized the volatile anesthetic desflurane to allow for repetitive imaging at sub-cellular resolution and re-identification of structures for up to a few days1.

Tags

In Vivo ImagingDrosophila LarvaeSub-cellular ResolutionOptical ImagingGenetically Encoded FluorophoresGenetic ToolsTransgenic Animal LinesAnesthetize LarvaeDesfluraneSynaptic PopulationsHigh Degree Of AnesthetizationSurvival RateTime-pointsProteins DynamicsGlutamate Receptor Subunit GluR-IIAEndogenous PromoterExon Trap Line FasII-GFP

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2249?article_type=v

High-resolution Measurement of Odor-Driven Behavior in Drosophila Larvae
check_url/2249?article_type=v

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging
check_url/2249?article_type=v

High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster
check_url/2249?article_type=v

Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila
check_url/2249?article_type=v

In-vivo Centrifugation of Drosophila Embryos
check_url/2249?article_type=v

Imaging Cell Shape Change in Living Drosophila Embryos
check_url/2249?article_type=v

Microgavage of Zebrafish Larvae
check_url/2249?article_type=v

Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis
check_url/2249?article_type=v

Ex vivo Method for High Resolution Imaging of Cilia Motility in Rodent Airway Epithelia
check_url/2249?article_type=v

Confocal Imaging of Single Mitochondrial Superoxide Flashes in Intact Heart or In Vivo

Read Article