Rat Pain Model
Rat Pain Model - Related Videos
01:37
A Preterm Rat Model for Pain Studies
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2024
Here, we present a concise protocol for creating a preterm rat model, facilitating research on early postnatal pain management. The method involves performing a cesarean section three days before the expected birth, extracting preterm rat pups via hysterectomy, and integrating them with the biological offspring of a surrogate dam.
04:39
Induction of Neuropathic Pain in a Rat Model by Constriction of the Infraorbital Nerve
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2025
Source: Deseure, K., et al. Chronic Constriction Injury of the Rat's Infraorbital Nerve (IoN-CCI) to Study Trigeminal Neuropathic Pain. J. Vis. Exp. (2015).This video demonstrates a method to induce neuropathic pain in a rat model by constricting the infraorbital nerve. After exposing the infraorbital nerve and tying ligatures to constrict it, the immune response at the injury site increases the excitability of neighboring neurons, which transmit pain signals to the brain.
02:02
Inducing Orofacial Neuropathic Pain in a Rat Model via Infraorbital Nerve Ligations
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2025
This video demonstrates the induction of orofacial neuropathic pain in a rat model by ligating the infraorbital nerve. This results in nerve damage and inflammation, causing abnormal pain signaling and mimicking chronic pain conditions, which can be used to study the orofacial pain assay.
10:52
Chronic Constriction Injury of the Rat's Infraorbital Nerve (IoN-CCI) to Study Trigeminal Neuropathic Pain
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Cited by 45 •
2015
This manuscript describes a rodent model to study trigeminal neuropathic pain. The methods described include surgical procedures to perform a chronic constriction injury of the rat’s infraorbital nerve and the post-surgical behavioral tests to measure the changes in spontaneous and evoked behavior that are indicative of persistent pain and allodynia.
08:23
Chronic Constriction of the Sciatic Nerve and Pain Hypersensitivity Testing in Rats
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Cited by 151 •
2012
Due to the simplicity of surgery and the robust behavioural outcome, chronic constriction of the sciatic nerve is one of the pre-eminent animal models of neuropathic pain. Within 24 hrs following surgery, pain hypersensitivity is established and can be quantitatively measured using a von Frey aesthesiometer (mechanical test) and plantar analgesia meter (thermal test).
FAQs
These models can be used to examine peripheral sensitization, in which responses become amplified near an affected tissue, and central sensitization, in which changes within the nervous system contribute to heightened sensitivity. Researchers relate these mechanisms to altered withdrawal, guarding, movement, or responses to touch and temperature, helping connect localized injury with broader changes in sensory processing.
Each behavior captures a different aspect of the animal’s response to a noxious condition. Withdrawal can indicate reactions to touch or temperature, while guarding and altered movement may reflect ongoing discomfort. Considering several outcomes helps researchers compare observable behavior with neural activity and reduces reliance on a single behavioral measure when investigating pain-related mechanisms.
A controlled stimulus or defined injury produces an observable response that can be interpreted alongside neural mechanisms. Changes in sensitivity, movement, guarding, or withdrawal provide behavioral outcomes for examining how sensory pathways interact with the nervous system. This connection allows neuroscience studies to investigate nociception while retaining a measurable link to whole-animal behavior.
A typical workflow selects a controlled noxious stimulus or defined injury, exposes the animal to that condition, and measures specified responses such as withdrawal, guarding, movement, or sensitivity to touch and temperature. Researchers then relate these outcomes to the biological question, such as sensitization, nociceptive processing, or the effects of an analgesic compound.
After establishing a defined pain-related condition, researchers can assess whether an analgesic compound changes measured outcomes, including withdrawal, guarding, movement, or sensitivity to touch and temperature. The model therefore provides a controlled basis for comparing treatment-related effects with pain-related behavior and for determining whether a compound influences the mechanisms under investigation.
They provide a setting in which researchers can examine pain mechanisms and test therapeutic strategies through measurable behavioral outcomes. Findings can connect nociception, neural activity, sensitization, and sensory pathways with treatment responses. This evidence supports the evaluation and refinement of analgesic approaches while showing how biological changes relate to observable pain-related behavior.