Sensory Neuron Transfection
Sensory Neuron Transfection - Related Videos
08:04
Neuronal Transfection Methods
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2023
Transfection - the process of transferring genetic material into cells - is a powerful tool for the rapid and efficient manipulation of gene expression in cells. Because this method can be used to silence the expression of specific proteins or to drive the expression of foreign or modified proteins, transfection is an extremely useful tool in the study of the cellular and molecular processes that govern neuron function. However, mature neurons have a number of properties that make them...
02:56
Electroporation-Mediated Transfection of Mouse Dorsal Root Ganglia to Promote Sensory Axon Regeneration
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2025
Source: Li, Q. et al. Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion. J. Vis. Exp. (2018)This video demonstrates electroporation-mediated transfection of mouse DRG neurons to study axon regeneration. It outlines the steps for delivering nucleic acid constructs to DRG neurons, inducing sciatic nerve injury, and assessing axonal regeneration.
17:27
Preparation of Aplysia Sensory-motor Neuronal Cell Cultures
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Cited by 37 •
2009
Primary cultures of Aplysia sensory-motor neurons provide a model preparation for studying synapse formation and synaptic plasticity in vitro. This video demonstrates the identification and microdissection of sensory and motor neurons from Aplysia ganglia as well as the methods for establishing and maintaining sensory-motor neurons in culture.
02:07
Stimulation of Dorsal Root Ganglion Sensory Neurons Using a Neuropeptide Receptor Agonist
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2025
This video demonstrates the use of transfected dorsal root ganglion cells to study neurotransmitter release by stimulating them with a neuropeptide receptor agonist.
06:56
Antibody Transfection into Neurons as a Tool to Study Disease Pathogenesis
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Cited by 10 •
2012
A rapid approach to investigate interactions and effects on molecular mechanisms related to the presence of antibodies in an intracellular environment is described. The method involves transfection of antibodies into live cells using a non-covalent complex formation based on a lipid formulation. The technique is adaptable to immortalized cell lines and primary cells.
FAQs
DNA-based constructs can drive production of a protein in the sensory neuron, allowing investigators to examine the consequences of increased or newly introduced protein activity. RNA-based material can instead suppress specific transcripts, reducing expression of selected gene products. This distinction lets researchers investigate gene function from complementary directions, including activation and inhibition of pathways involved in sensory signaling.
Lipid-based reagents and viral vectors serve as delivery systems that transport genetic material across the neuronal membrane. Their central role is to make DNA, RNA, or related constructs accessible inside sensory neurons, where the payload can influence gene activity. Comparing these systems is relevant when planning experiments that require either protein expression or suppression of specific transcripts.
Sensory neurons detect touch, temperature, pain, and other stimuli, so altering their gene activity can connect specific molecular changes with sensory functions. Transfected cells provide a focused setting for examining ion channels, receptors, and neuronal signaling. This helps researchers investigate how these components contribute to pain mechanisms and other sensory processes.
The available payloads include DNA, RNA, and related genetic constructs. DNA can be used when the experiment aims to drive protein expression, whereas RNA can support suppression of selected transcripts. The appropriate material therefore depends on whether the study seeks to add or monitor a gene product, or reduce activity from a particular transcript.
A basic workflow begins by selecting a genetic payload that matches the experimental objective, such as protein expression or transcript suppression. Researchers then pair that material with a delivery system, such as a lipid-based reagent or viral vector, to introduce it into sensory neurons. Subsequent analysis focuses on altered gene activity and its effects on neuronal signaling or related functions.
Researchers can assess whether the introduced material changes protein expression or suppresses a selected transcript, then relate those molecular effects to sensory-neuron behavior. Relevant outcomes include changes in ion-channel or receptor function, neuronal signaling, and pain-related mechanisms. These observations also support evaluation of gene-regulation strategies and exploration of potential treatments for sensory disorders.