Stage Scanning
Stage Scanning - Related Videos
02:28
Leaky Scanning
0 Views •
2020
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
11:41
Scanning Electron Microscopy (SEM)
0 Views •
2023
Source: Laboratory of Dr. Andrew J. Steckl — University of Cincinnati A scanning electron microscope, or SEM, is a powerful microscope that uses electrons to form an image. It allows for imaging of conductive samples at magnifications that cannot be achieved using traditional microscopes. Modern light microscopes can achieve a magnification of ~1,000X, while typical SEM can reach magnifications of more than 30,000X. Because the SEM doesn’t use light to create images, the resulting pictures it...
12:03
Differential Scanning Calorimetry
0 Views •
2023
Source: Danielle N. Beatty and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Differential scanning calorimetry (DSC) is an important measurement to characterize thermal properties of materials. DSC is used primarily to calculate the amount of heat stored in a material as it heats up (heat capacity) as well as the heat absorbed or released during chemical reactions or phase changes. However, measurement of this heat can also lead to...
14:10
Video-rate Scanning Confocal Microscopy and Microendoscopy
0 Views •
Cited by 14 •
2011
The complete construction of a custom, real-time confocal scanning imaging system is described. This system, which can be readily used for video-rate microscopy and microendoscopy, allows for an array of imaging geometries and applications not accessible using standard commercial confocal systems, at a fraction of the cost.
01:22
Stages of Sleep
0 Views •
2024
Sleep progresses through distinct stages, each characterized by specific brain wave patterns and physiological responses ranging from wakefulness to stages of non-rapid eye movement, known as non-REM, to rapid eye movement, referred to as REM. Understanding these stages helps in recognizing how sleep supports various bodily and cognitive functions. Before sleep begins, in wakefulness, the brain exhibits primarily beta waves, which are high in frequency and low in amplitude, indicating alertness...
FAQs
Overlapping images provide shared spatial information between neighboring acquisitions, allowing the recorded fields to be aligned and combined into a continuous mosaic. This arrangement helps preserve the relationships among structures across image boundaries rather than treating each field as an isolated observation. The resulting mosaic supports examination of extensive tissue regions within one spatially coherent representation.
Controlled stage movements establish a consistent sequence of specimen positions during acquisition. Because the stage advances in defined steps while the imaging system records successive fields, researchers can systematically sample a large specimen and repeat the acquisition approach across samples. This reproducibility is especially valuable for quantitative neuroanatomy, where spatially organized measurements depend on consistent coverage.
A single field of view captures only a limited portion of a specimen, whereas sequential stage positions provide coverage across a larger area. The microscope records images as the specimen moves through the fixed imaging system, creating either a mosaic after alignment or a spatial series for position-by-position analysis. This enables broader sample surveys without discarding spatial organization.
The workflow begins by positioning the specimen within the fixed imaging system and defining a sequence of stage movements across the region of interest. The motorized stage then advances in controlled steps, and the microscope records successive, overlapping images. After acquisition, the images can be aligned and combined into a mosaic or retained as a spatial series for analysis.
Neuroscientists can apply this approach when the region of interest extends beyond one microscope field, such as in brain tissue, neural circuits, or histological sections. Systematic scanning allows researchers to survey extensive samples while maintaining spatial context. That combination supports investigations requiring broad anatomical coverage rather than observations limited to a small, selected field.
Stage Scanning can produce aligned mosaics that represent broad tissue regions, or spatial series that preserve the order and location of successive images. These outputs support systematic examination of neural anatomy and circuits across extensive samples. In neuroscience, the method is relevant to quantitative neuroanatomy and large-scale microscopy because automated acquisition can improve consistency during broad image collection.