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Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

Article DOI: 10.3791/1350-v • 07:47 min • August 14th, 2009
August 14th, 2009 •
1, 1
1Protein Expression and Purification Core Facility, EMBL Heidelberg

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A short protocol for protein staining with Coomassie Brilliant Blue (CBB) G-250 in polyacrylamide gels is described without using organic solvents or acetic acid as in the classical staining procedures with CBB.

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Proteins Gels Coomassie G-250 Staining Organic Solvent Acetic Acid SDS-PAGE Microwave Oven Toxic Solvents Safety Considerations Alternative Composition Bidistilled Water HCl Washing Heating Gel Protein Staining
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