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Isolation of Limbal Niche Cells (LNCs) from Limbus Tissue (Video) | JoVE

A Protocol to Isolate, Culture, and Identify Human Limbal Niche Cells

Article DOI: 10.3791/200727-v • 05:24 min • October 27th, 2023
October 27th, 2023 •
1, 1, 1, 1, 1, 1, 1, 1, 2, 3, 1
1Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 2Institute of Medicine Nursing, Hubei University of Medicine, 3Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology

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Cellules de niche limbique Isolement Identification Tissu limbique Amas cellulaires Milieu de cellules souches embryonnaires Matrigel Immunofluorescence PCR quantitative en temps réel Cytométrie en flux Marqueurs de cellules souches VIM CD90 CD105 PDGFRβ Pan-CK CD73 SCF BMMSCs
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