JoVE Neuroscience is a multidisciplinary section devoted to investigations of the structure, function, physiology, and pathophysiology of the brain and nervous system. Included methodologies range from molecular and cellular level studies to full central and peripheral neural systems. Potential treatment platforms and surgical techniques for neurological diseases and disorders are also presented in this section.

Neuroscience

JoVE Medicine serves as a connection between basic research and clinical applications. Articles may present medical procedures, case studies or clinical trial methodologies. Included studies are often modeled towards predictive, preventative, personalized, and practical approaches to treatment of disease. The goal of research methodologies in this section is to improve overall patient care and prognosis.

Medicine

JoVE Immunology and Infection includes content from a variety of fields working toward a shared initiative of improving global health. Techniques evaluating the biological response to pathogens from the molecular to organismal level are presented as well as therapeutic agents and their efficacy in treating disease.

Immunology and Infection

JoVE Genetics publishes video articles demonstrating experimental approaches to investigate gene function and regulation. This includes methods to study gene expression, mutagenesis, epigenetics, genetic determinants of development, genetic disorders, population genetics and evolution.

Genetics

JoVE Environment is dedicated to research methodologies that address environmental concerns and seek to better understand Earth's ecosystem.  Special consideration is given to experimental methodologies that assess society’s impact on the environment, suggest solutions for protecting Earth’s resources, and develop sustainable fuel sources. Moving towards an environmentally conscious planet is a collaborative effort with contributions from both life and physical scientists.

Environment

JoVE Engineering encompasses a broad range of experimental and instrumental techniques utilized in physics research. Investigations in this area strive to address and answer a broad range of scientific questions, such as device mechanisms and efficiencies, using physical tools. This approach often requires a combination of specialties, and research in this area tends to be interdisciplinary with contributions from mechanical, electrical, and chemical engineers.

Engineering

JoVE Developmental Biology publishes research methodologies in the field of developmental biology. This includes methods that are used to study biological development in vitro and in vivo at the molecular, cellular, tissue, organ and whole organism levels.

Developmental Biology

JoVE Chemistry surveys a broad scope of chemical disciplines. This collection includes fundamental and applied research that investigates molecular interactions, properties, and structure spanning in the following core divisions: analytical, organic, organometallic, inorganic, bioinorganic, and biochemistry. This section also includes the design and preparation of materials and small molecules for advanced or unique applications.

Chemistry

JoVE Cancer Research illustrates experimental approaches in biomedical research and clinical practice aimed at understanding, detecting, treating and preventing cancer. The video articles in this section demonstrate methods to study the cellular and molecular mechanisms of cancer, animal models of cancer research, development of new therapies and diagnostics, tumor imaging, chemical and radiation treatments, surgical procedures and improvements in therapy. These video articles offer researchers and clinical practitioners an efficient way to learn the technologies and experimental methods in cancer research.

Cancer Research

JoVE Biology welcomes all general biology research methodologies. Content in this section canvases all fields of cell, molecular, and organismal biology, ranging from new applications of standard techniques to novel approaches aimed at understanding the functions of life and living organisms. This diverse section includes, but is not limited to, techniques in physical biology, cellular biochemistry, genetics, physiology, systems biology and a combination of eukaryotic and prokaryotic model systems.

Biology

JoVE Bioengineering merges both physical and life sciences to understand and predict biological processes. Applying physical science tools to life science questions allow for the discovery of better technologies to measure, diagnose, and clinically treat disease.

Bioengineering

JoVE Biochemistry publishes experiments that investigate the structure, function, and interactions of biological molecules. The video articles in this section demonstrate methods to study nucleic acids, proteins, carbohydrates and lipids as well as enzyme function, protein folding, molecular interactions and biochemical reactions.

Biochemistry

JoVE Behavior documents observational and experimental techniques that seek to understand human and animal behavior. Work in this field includes analysis of genetic causes and physiological responses to internal or external stimuli as well as the implicated brain regions.

Behavior

Este protocolo presenta un enfoque contralateral axilo-bilateral-mama para tiroidectomía endoscópica que optimiza la posición quirúrgica y el espacio operatorio, logrando excelentes resultados estéticos.

Un novedoso enfoque contralateral axilo-bilateral-mamario para cirugía endoscópica de tiroides

Este protocolo demuestra la ablación láser transvesical Ho:YAG para crear un modelo reproducible en rata de fibrosis del cuello de la vejiga para estudiar la remodelación fibrótica inducida por lesión térmica y las intervenciones terapéuticas.

Inducción asistida por láser de la contractura del cuello de la vejiga en un modelo de rata

El Cuerpo Vítreo Capsular Plegable (FCVB) es un implante intraocular que encapsula aceite de silicona dentro de una cápsula flexible para reducir las complicaciones relacionadas con el aceite. Este protocolo describe la implantación quirúrgica de la FCVB mediante incisión escleral para el tratamiento del desprendimiento complejo de retina y el traumatismo ocular.

Protocolo de implantación del cuerpo vítreo capsular plegable para cirugía vítreoretiniana compleja

Aquí presentamos un protocolo integrado para generar lesiones osteocondrales controladas en rodillas de oveja con el fin de establecer un modelo experimental adecuado para estudios de regeneración de tejido óseo y cartílago. El protocolo describe un método para inducir un defecto osteocondral de grosor completo en el cóndilo femoral, utilizando técnicas que minimizan los impactos en el bienestar animal y la variabilidad en los resultados.

Establecimiento y evaluación de un modelo ovino de defecto osteocondral de espesor completo

Presentamos un modelo reproducible de ratón de alotrasplante compuesto vascularizado de extremidades posteriores a cuello heterotópico. Este protocolo supermicroquirúrgico, que implica anastomosis de vasos de 0,3-0,5 mm, integra guía con filamento de nailon y esposas venosas. Proporciona una plataforma fiable para estudios traslacionales sobre tolerancia, lesiones por isquemia-reperfusión y mecanismos inmunológicos en alotrasplantes compuestos vascularizados.

Trasplante heterotópico de extremidad posterior a cuello en ratón: un modelo supermicroquirúrgico de alotrasplante compuesto vascularizado

Este estudio describe un procedimiento quirúrgico estandarizado para la disección endoscópica de ganglios axilares de un solo puerto sin liposucción en el cáncer de mama. Indicada para pacientes con metástasis ganglional confirmada por biopsia o ganglios linfáticos centinela positivos, la técnica enfatiza la preservación de estructuras críticas, especialmente el nervio intercostobraquial, para reducir la morbilidad postoperatoria y optimizar la recuperación funcional.

Disección endoscópica de ganglios linfáticos axilares sin liposucción de un solo puerto en cirugía de cáncer de mama

Este informe de caso en vídeo demuestra el manejo único de un tumor estromalical duodenal gastrointestinal con una rara y poco estudiada mutación del exón 8 KIT. La terapia neoadyuvante resultó en una reducción del 30% en el tamaño tumoral, convirtiendo al paciente en candidato para una resección duodenal asistida por robot en la manga en lugar de la más mórbida pancreaticoduodenectomía.

Resección robótica de manga duodenal para tumor estromal gastrointestinal con mutación rara del exón 8 KIT tras el neoadyuvante imatinib

Los organoides cardíacos humanos autoensamblantes (hCOs) son un sistema emergente para modelar el desarrollo y las enfermedades cardíacas humanas. Aquí se proporciona una metodología para generar y dañar hCOs mediante hipoxia-reoxigenación y detalles de los resultados histológicos y funcionales esperados tras la lesión.

Un modelo de lesión por hipoxia-reoxigenación en cardioides humanos autoensamblantes

Este protocolo presenta una técnica de trasplante cardíaco heterotópico cervical sin manguito y basada en sutura, diseñada para permitir la colocación fiable de un segundo injerto cardíaco heterotópico para evaluar la tolerancia inmunitaria específica del donante bajo condiciones fisiológicas con mínima interferencia de material extraño.

Modelo cervical basado en suturas sin manguito para trasplante cardíaco heterotópico secundario con el fin de evaluar la tolerancia inmunitaria específica del donante en ratones

Presentamos un protocolo detallado para establecer y manipular genéticamente organoides cervicales humanos.

Journal - Medicine