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Preparation of Plate for Fluorescent Imaging and Cell Viability Assessment in CAR-Jurkat Co-Culture with CFSE-Labeled Tumor Cells (Video) | JoVE

Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells

Article DOI: 10.3791/200793-v • 04:00 min • October 27th, 2023
October 27th, 2023 •
*1,2,3, *1, 4, 2, 1, 4,5,6, 1,2,3
1Department of Radiation Oncology, University of Kansas Cancer Center, 2Department of Cancer Biology, University of Kansas Cancer Center, 3BioEngineering Program, University of Kansas, 4Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, 5University of Kansas Cancer Center, 6Kansas Institute for Precision Medicine, University of Kansas Medical Center
* These authors contributed equally

Tags

Rapid In Vitro Cytotoxicity Evaluation Jurkat Chimeric Antigen Receptor Fluorescent Imaging CAR T Cells Targeting Domain Single Chain Variable Fragment Intra-chain Linker Hinge Domain Transmembrane Domain Costimulatory Domain CAR Construct CAR-mediated Killing CAR-T Cells Time-consuming Process Expensive Process Testing Constructs Time And Material Investment Platform Hinge-optimized CAR Constructs Jurkat Cells Lentivirus Uptake CAR Transduction Fluorescent Imager Cytolysis PBMC-derived T Cells
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