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JoVE Journal Neuroscience
Antibody Transfection into Neurons as a Tool to Study Disease Pathogenesis
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Antibody Transfection into Neurons as a Tool to Study Disease Pathogenesis

Article DOI: 10.3791/4154-v • 06:56 min • September 26th, 2012
September 26th, 2012 •
1,2,3, 1,2, 1,2, 1,2, 1,2, 1,2,3
1Research Service, Veterans Administration Medical Center, Memphis, TN, 2Department of Neurology, University of Tennessee Health Science Center, Memphis, TN, 3Department of Anatomy/Neurobiology, University of Tennessee Health Science Center, Memphis, TN

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Resumen
Automatic Translation

A rapid approach to investigate interactions and effects on molecular mechanisms related to the presence of antibodies in an intracellular environment is described. The method involves transfection of antibodies into live cells using a non-covalent complex formation based on a lipid formulation. The technique is adaptable to immortalized cell lines and primary cells.

Tags

Antibody Transfection Neurons Study Disease Pathogenesis Specific Antibodies Human Diseases Systemic Lupus Erythematosus Rheumatoid Arthritis Paraneoplastic Syndromes Multiple Sclerosis HTLV-1 Myelopathy/tropical Spastic Paraparesis Inflammation Cellular Messaging Apoptosis Intracellular RNA Binding Protein Heterogeneous Ribonuclear Protein A1 (hnRNP A1) Pathogenic Antibodies Intracellular Antibody:protein Interactions Transfection Of Antibodies Alteration Of Antibody Structure Poor Transfection
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