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A Simple Protocol for Extracting Hemocytes from Wild Caterpillars
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A Simple Protocol for Extracting Hemocytes from Wild Caterpillars

Article DOI: 10.3791/4173-v • 07:35 min • November 15th, 2012
November 15th, 2012 •
1, 1, 1, 1
1Department of Biological Sciences, The George Washington University

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Insect hemocytes carry out many important functions, both immune and non-immune, throughout all stages of insect development. Our present knowledge of hemocyte types and function comes from studies on insect genetic models. Here, we present a method for extracting, quantifying and visualizing hemocytes from wild caterpillars.

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Hemocytes Wild Caterpillars Insect Immune Response Lymph Gland Embryonic Hemocytes Larval Stages Insect Innate Immune Response Cell Migration Chemotaxis Regulation Cell Apoptosis Embryogenesis Cell Spreading Cell Aggregation Nodules Formation Phagocytosis Encapsulation Antimicrobial Peptides Effector Molecules Hemocyte Morphology Hemocyte Function Non-model Insect Species
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