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JoVE Journal Behavior
Use of the Operant Orofacial Pain Assessment Device (OPAD) to Measure Changes in Nociceptive Behavior
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Use of the Operant Orofacial Pain Assessment Device (OPAD) to Measure Changes in Nociceptive Behavior

Article DOI: 10.3791/50336-v • 12:20 min • June 10th, 2013
June 10th, 2013 •
1,2, 3, 4, 4, 1, 3, 1,2, 4
1Department of Oral and Maxillofacial Surgery, University of Florida College of Dentistry, 2Department of Neuroscience, McKnight Brain Institute, University of Florida College of Medicine, 3Stoelting Co., 4Department of Orthodontics, University of Florida

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We present a user-friendly, high-throughput operant system for the evaluation of pain behaviors in awake, conscious rodents. The Orofacial Pain Assessment Device (OPAD) can assess pain through a reward/conflict paradigm thus providing a more humane way of testing. This protocol will yield more clinically relevant and translational data from rodents.

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Operant Orofacial Pain Assessment Device OPAD Pain Detection Nociceptive Behavior Pain Behaviors Reflex-based Measures Clinically Relevant Peltier-based Thermodes Temperature Programming Reward Bottle Behavioral Pain Outcomes Reward Bottle Activations Lick/face Ratio Stimulus Temperature High-throughput Operant Assay Cortical Input Spinal Reflex-based Nociceptive Assays
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