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Ex vivo Method for High Resolution Imaging of Cilia Motility in Rodent Airway Epithelia
JoVE Revista
Biología
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JoVE Revista Biología
Ex vivo Method for High Resolution Imaging of Cilia Motility in Rodent Airway Epithelia

Ex vivo Method for High Resolution Imaging of Cilia Motility in Rodent Airway Epithelia

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08:25 min

August 08, 2013

DOI:

08:25 min
August 08, 2013

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Summary

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An easy and reliable technique for visualizing and quantifying airway cilia motility and cilia generated flow using mouse trachea is described. This technique can be modified to determine how a wide range of factors influence cilia motility, including pharmacological agents, genetic factors, environmental exposures, and/or mechanical factors such as mucus load.

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