Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
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January 08, 2014
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Compared with traditional affinity chromatography using protein A agarose bead-packed columns, protein A membrane adsorbers can significantly speed laboratory-scale isolation of antibodies and other Fc fragment-expressing proteins. Appropriate analysis and quantification methods can further accelerate protein processing, allowing isolation/characterization to be completed in one workday, instead of 20+ work hours.
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Burdick, M. M., Reynolds, N. M., Martin, E. W., Hawes, J. V., Carlson, G. E., Cuckler, C. M., Bates, M. C., Barthel, S. R., Dimitroff, C. J. Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow. J. Vis. Exp. (83), e51023, doi:10.3791/51023 (2014).
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