Journal
/
Neurociencias
/
Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

14,047 Views

•

10:33 min

•

May 22, 2014

DOI:

10.3791/51609-v

DOI:
10.3791/51609-v

•

10:33 min
May 22, 2014

•

38 Views
, , ,
1Developmental Biology, Center for Stem Cell Biology,Sloan-Kettering Institute for Cancer Research, 2St. Giles Laboratory of Human Genetics of Infectious Diseases,The Rockefeller University

Summary

Automatically generated

Neural crest (NC) cells derived from human pluripotent stem cells (hPSC) have great potential for modeling human development and disease and for cell replacement therapies. Here, a feeder-free adaptation of the currently widely used in vitro differentiation protocol for the derivation of NC cells from hPSCs is presented.

Explore More Videos

Feeder-freeNeural Crest Progenitor CellsHuman Pluripotent Stem CellsIn Vitro DifferentiationEmbryonic DevelopmentPeripheral Nervous SystemMelanocytesMesenchymal CellsRegenerative MedicineDisease Modeling

Read Article

Cite this Article

Zeltner, N., Lafaille, F. G., Fattahi, F., Studer, L. Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells. J. Vis. Exp. (87), e51609, doi:10.3791/51609 (2014).

Download .ris file

Copy

Share Video

.

Copy