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Immunology and Infection

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Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

Article DOI: 10.3791/51663-v • 13:29 min • May 28th, 2014
May 28th, 2014 •
1, 1, 1, 1, 1,2,3, 1,3
1Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead Hospital, 2Centre for Research Excellence in Critical Infection, Westmead Millennium Institute, Westmead Hospital, 3Sydney Emerging Infectious Diseases Institute, University of Sydney, Westmead Hospital

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Resumen
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The application of matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) directly to blood culture broth expedites the identification of bacteria. The presented method is a rapid and reliable method for identification of Gram negative bacteria directly from blood culture broth.

Tags

MALDI-TOF Mass Spectrometry Gram-negative Bacteria Blood Culture Broth Rapid Identification Clinical Microbiology Laboratory Sub-culture Solid Agar Species Identification Clinical Management Interfering Resins Proteins Hemoglobin MS Spectra Discrimination Identification Scores
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