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Immunology and Infection

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Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Article DOI: 10.3791/52505-v • 16:01 min • January 26th, 2015
January 26th, 2015 •
1,2, 3, 2, 1
1Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, 2Division of Allergy and Immunology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University

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The goal of the present protocol was to develop a method that will allow functional genomic analyses of mast cell secretion. The protocol is based on quantitative assessment of the release of a fluorescent reporter gene cotrasfected with the gene of interest and real time analyses of the secretory granule's morphology.

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Mast Cells Secretory Granules Biosynthesis Exocytosis Allergic Mediators Inflammatory Mediators Immunoregulatory Mediators Immune Responses Cytoplasmic Secretory Granules Regulated Exocytosis Gene Expression Reporter Gene High Resolution Confocal Analyses Biogenesis Triggered Exocytosis Molecular Mechanisms Regulators Cellular Mechanisms Health And Disease
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