Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Chemistry / Dissolution Dynamic Nuclear Polarization…
JoVE Journal
Chemistry

Se requiere una suscripción a JoVE para ver este contenido.

JoVE Journal Chemistry
Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
Efficient Construction of Drug-like Bispirocyclic Scaffolds Via Organocatalytic Cycloadditions of α-Imino γ-Lactones and Alkylidene Pyrazolones
Journal (Chemistry)
Efficient Construction of Drug-like Bispirocyclic Scaffolds Via Organocatalytic Cycloadditions of α-Imino γ-Lactones and Alkylidene Pyrazolones
The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes
Journal (Chemistry)
The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes
 

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Article DOI: 10.3791/53548-v • 10:54 min • February 23rd, 2016
February 23rd, 2016 •
1, 1, 2, 3, 3, 1
1LCBPT - UMR8601, Institut de Chimie, Université Paris Descartes, 2Institute of Physics of Biological Systems, EPFL, 3PARCC - INSERM U970, Université Paris Descartes

Capítulos

Resumen
Automatic Translation

The sensitivity enhancement provided by dissolution dynamic nuclear polarization (DNP) enables following metabolic processes in real time by NMR and MRI. The characteristics and performances of a dedicated dissolution DNP setup designed for study enzymatic reactions are discussed.

Tags

Dissolution Dynamic Nuclear Polarization Enzymatic Reaction Rate Measurements NMR Dynamic Nuclear Polarization DNP Equipment Real-time Enzymatic Processes Liquid-state Magnetic Resonance Pair Of Molecules Dissolution DNP Equipment Sensitivity Enhancement 500 Megahertz NMR Spectrometers France's First DNP Equipment Installation Substrate Quantification Time Resolution
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter