Novel Approach for Simultaneous Recording of Renal Sympathetic Nerve Activity and Blood Pressure with Intravenous Infusion in Conscious, Unrestrained Mice.

Shereen M. Hamza; John E. Hall

doi:10.3791/54120

Nuevo enfoque para la grabación simultánea de la actividad nerviosa simpática Renal y presión art...

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Novel Approach for Simultaneous Recording of Renal Sympathetic Nerve Activity and Blood Pressure with Intravenous Infusion in Conscious, Unrestrained Mice.

Nuevo enfoque para la grabación simultánea de la actividad nerviosa simpática Renal y presión arterial con la infusión intravenosa en consciente, libre de ratones.

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11:08 min

February 14, 2018

DOI: 10.3791/54120-v

Shereen M. Hamza¹, John E. Hall²

¹Department of Pediatrics,University of Alberta, ²Department of Physiology & Biophysics,University of Mississippi Medical Center, ³Mississippi Center for Obesity Research

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Overview

This article outlines a novel method for assessing renal sympathetic nerve activity in conscious mice, avoiding the confounding effects of anesthesia. This technique allows for reliable evaluation of autonomic tone in a more physiologically relevant setting.

Key Study Components

Area of Science

Neuroscience
Physiology
Experimental Biology

Background

Anesthetized mice show non-physiological blood pressure.
Blood pressure is closely linked to autonomic nervous system activity.
Conscious models provide more accurate assessments of nerve activity.
Recent interest in mouse models necessitates improved methodologies.

Purpose of Study

To evaluate renal sympathetic activity in conscious mice.
To eliminate the confounding effects of anesthesia.
To provide a reliable technique for assessing autonomic tone.

Methods Used

Surgical preparation of the mouse.
Positioning the mouse for surgery.
Making incisions for nerve activity assessment.
Simultaneous recording of nerve activity and blood pressure.

Main Results

Successful assessment of renal sympathetic nerve activity in conscious mice.
Demonstrated reliability of the technique without anesthesia.
Provided insights into autonomic tone in a resting state.

Conclusions

This method allows for accurate evaluation of autonomic function.
Conscious models enhance the understanding of renal sympathetic activity.
The technique is accessible for researchers interested in autonomic studies.

Frequently Asked Questions

What is the significance of studying renal sympathetic nerve activity?

Understanding renal sympathetic nerve activity is crucial for assessing autonomic regulation and its impact on blood pressure.

How does anesthesia affect the assessment of autonomic tone?

Anesthesia can alter blood pressure and nerve activity, leading to inaccurate assessments of autonomic tone.

What advantages does this method offer over traditional techniques?

This method allows for assessments in conscious mice, providing more physiologically relevant data without the effects of anesthesia.

Is this technique applicable to other animal models?

While this study focuses on mice, the principles may be adapted for other conscious animal models.

What preparations are needed before the surgical procedure?

Mice must be properly anesthetized for surgery, and the surgical area should be cleaned and sterilized.

Can this method be used to study other aspects of the autonomic nervous system?

Yes, the technique can potentially be adapted to study various autonomic functions beyond renal sympathetic activity.

Ratones anestesiados exhiben la presión arterial sistémica no fisiológico, que impide una evaluación significativa del tono autonómico, dada la íntima relación entre la presión arterial y el sistema nervioso autónomo. Así, se describe un método novedoso para la actividad del nervio simpático renal al mismo tiempo récord y la presión arterial con la infusión intravenosa en ratones conscientes.

El objetivo general de este novedoso método quirúrgico y experimental es evaluar la actividad simpática renal en ratones conscientes y sin restricciones sin los efectos confusos de la anestesia. Dado el reciente interés en los modelos de ratón, este enfoque proporciona una técnica accesible mediante la cual el tono autónomo puede evaluarse de forma fiable en ratones conscientes. La principal ventaja de esta técnica es que se puede evaluar la actividad nerviosa en el ratón consciente y en reposo sin factores de confusión como la anestesia o la restricción.

Después de preparar al ratón para la cirugía, colóquelo sobre su lado derecho con el extremo rostral apuntando hacia la izquierda del cirujano. Primero, haz una incisión de cinco milímetros en la región media dorsal con el bisturí. En segundo lugar, hacer una incisión más grande en la piel que recubre el flanco izquierdo perpendicular a la columna vertebral en dos milímetros caudal a la caja torácica.

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