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Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks

Article DOI: 10.3791/54678-v • 12:19 min • November 10th, 2016
November 10th, 2016 •
1, 1
1Division of Pharmacology and Toxicology, College of Pharmacy, The University of Texas at Austin

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Targeted DNA damage can be achieved by tethering a DNA damaging agent to a triplex-forming oligonucleotide (TFO). Using modified TFOs, DNA damage-specific protein association, and DNA topology modification can be studied in human cells by the utilization of modified chromatin immunoprecipitation assays and DNA supercoiling assays described herein.

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Architectural Protein HMGB1 DNA Damage DNA Repair Site-specific DNA Damage Chemotherapeutic Agents Carcinogenic Agents Chromatin Immunoprecipitation DNA Supercoiling Pharmacological Targets Cancer Therapy Transfection Mammalian Cells Transfectionary Agent TFO-directed ICL-containing Plasmids Growth Medium PBS
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