Minimally Invasive Transverse Aortic Constriction in Mice

Aung Moe Zaw; Connor M. Williams; Helen K. W. Law; Billy Kwok Chong Chow

doi:10.3791/55293

La constricción aórtica mínimamente invasiva transversal en ratones

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Minimally Invasive Transverse Aortic Constriction in Mice

La constricción aórtica mínimamente invasiva transversal en ratones

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07:40 min

March 14, 2017

DOI: 10.3791/55293-v

Aung Moe Zaw¹, Connor M. Williams², Helen K. W. Law³, Billy Kwok Chong Chow¹

¹School of Biological Sciences,The University of Hong Kong, ²College of Arts and Science,New York University, ³Department of Health Technology and Informatics, Faculty of Health and Social Sciences,The Hong Kong Polytechnic University

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Overview

This article discusses a minimally invasive technique known as transverse aortic constriction (MTAC) that induces left ventricular hypertrophy without the need for artificial ventilation. This method is particularly beneficial for high-throughput studies on left ventricular overload.

Key Study Components

Area of Science

Cardiology
Cardiac hypertrophy
Myocardial remodeling

Background

Left ventricular hypertrophy is a key area of research in cardiology.
Traditional methods often require artificial ventilation.
MTAC offers a less invasive alternative.
This technique can facilitate translational studies.

Purpose of Study

To induce left ventricular hypertrophy effectively.
To study myocardial remodeling and related conditions.
To eliminate the need for tracheal intubation in the procedure.

Methods Used

Use of curved 30-gauge needles for constriction.
Preparation of a 27-gauge needle with a bent tip.
Application of a spacer for precise constriction.
Conducting the procedure without artificial ventilation.

Main Results

MTAC successfully induces left ventricular hypertrophy.
Eliminates the need for ventilators.
Facilitates high-throughput studies in cardiology.
Provides insights into cardiac hypertrophy and fibrosis.

Conclusions

MTAC is a desirable method for studying left ventricular overload.
It conserves the essentials of traditional TAC.
This technique can enhance translational research in cardiology.

Frequently Asked Questions

What is MTAC?

MTAC stands for minimally invasive transverse aortic constriction, a method to induce left ventricular hypertrophy without artificial ventilation.

Why is MTAC preferred over traditional TAC?

MTAC eliminates the need for tracheal intubation and artificial ventilation, making it less invasive and more suitable for high-throughput studies.

What are the main applications of MTAC?

MTAC is used to study myocardial remodeling, cardiac hypertrophy, and related conditions in a translational research context.

How is the MTAC procedure performed?

The procedure involves using curved needles to constrict the aortic arch, inducing left ventricular hypertrophy without the need for ventilation.

What are the benefits of using MTAC?

MTAC allows for effective induction of hypertrophy while simplifying the surgical process and enhancing research capabilities.

Can MTAC be used for high-throughput studies?

Yes, MTAC is particularly desirable for high-throughput studies on left ventricular overload.

Mínimamente invasiva constricción de la aorta transversal (MTC) conserva los elementos esenciales de la constricción de la aorta transversal regular (TAC) al tiempo que elimina el uso de un ventilador con intubación traqueal. Demuestra ser un método altamente deseable para los estudios de alto rendimiento de sobrecarga ventricular izquierda, sobre todo en estudios de traducción.

El objetivo general de este procedimiento es inducir la hipertrofia ventricular izquierda mediante la constricción del arco aórtico transversal con un procedimiento quirúrgico simple que no requiere ventilación artificial. Este método puede ayudar a responder preguntas clave en el campo de la cardiología sobre la remodelación miocárdica, la hipertrofia cardíaca, la apoptosis y la fibrosis, y las condiciones de sobrecarga de presión ventricular izquierda. La principal ventaja de esta técnica es que conserva los elementos esenciales de la constricción aórtica transversa regular, al tiempo que elimina la necesidad de un ventilador y la intubación traqueal.

Antes de comenzar el procedimiento, use un portaagujas para curvar las puntas de dos a cuatro agujas de calibre 30 y monte las agujas en aplicadores de algodón individuales. A continuación, desafila una aguja de calibre 27 y utiliza el portaagujas para doblar la punta 90 grados. A continuación, alisa la punta sobre una superficie dura para hacer un espaciador de 0,4 milímetros.

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