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Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins
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Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

Article DOI: 10.3791/55312-v • 08:14 min • February 25th, 2017
February 25th, 2017 •
1, 1
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School

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We describe sensitive, gel-based discontinuous assays to examine the kinetics of lagging-strand initiation using the replication proteins of bacteriophage T7.

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Kinetics Lagging-strand DNA Synthesis In Vitro Bacteriophage T7 Replication Proteins DNA Replication Primer Synthesis Lagging Strand Synthesis Okazaki Fragment Synthesis Radioactive Materials Formamide Dye Buffer Time Points ATP CTP P32 Labeling Single-strand DNA Template Gene Four Protein GP Four Primase Helicase Incubation Reaction Control Magnesium Chloride Timer
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