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Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells
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Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells

Article DOI: 10.3791/55687-v • 09:16 min • April 20th, 2017
April 20th, 2017 •
*1, *1, 1, 1, 1
1Metabolic Research Laboratories and MRC Metabolic Diseases Unit, Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge
* These authors contributed equally

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This protocol describes the isolation and culture of mixed murine small intestinal cells. These primary intestinal cultures enable the investigation of signaling pathways underlying gut peptide secretion using a number of techniques.

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Mixed Primary Cultures Murine Small Intestine Gut Hormone Secretion Live Cell Imaging Enteroendocrine Cells In Vitro Characterization Intestinal Epithelium Genetic Fluorescent Tagging Single-cell Electrophysiological Cheryl Brighton Mouse Duodenum Pasteur Pipette Chilled PBS Adiposed Tissues Mesentary Dissecting Microscope
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