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Developmental Biology

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JoVE Journal Developmental Biology
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Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks

Article DOI: 10.3791/57099-v • 09:33 min • March 29th, 2018
March 29th, 2018 •
1, 1, 2, 1,2, 2, 1, 3, 3, 3
1The Institute for Basic Research, The New York State Office for People with Developmental Disabilities, 2Placental Analytics LLC, 3Visikol Inc.

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This study presents a protocol for the reversible tissue clearing, immunostaining, 3D-rendering and analysis of vascular networks in human placenta villi samples on the order of 1 - 2 mm3.

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Three-dimensional Rendering Analysis Immunolabeled Clarified Human Placental Villous Vascular Networks Growth Restricted Pre-term Birth Immunolabeling Visualizations Characterize Analyze Histological Characterization Diagnoses Therapies Ms. Valerie Schwenk Mr. Phillip Necaise Dissection Microscope Petri Dish Scalpel Forceps
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