Detección de la pequeña GTPasa Prenilación y GTP obligatorio usar membrana fraccionamiento y GTPasa-linked Immunosorbent Assay

9.6K visualizaciones

Citado por 6

13:51 min.

11 de noviembre de 2018

10.3791/57646-v

11 de noviembre de 2018

9.6K visualizaciones

Javad Alizadeh¹^,² , Shahla Shojaei¹^,²^,³ , Simone da Silva Rosa¹ , Adel Rezaei Moghadam¹^,² , Amir A. Zeki⁴ , Mohammad Hashemi⁵ , Marek J. Los⁶^,⁷^,⁸ , Joseph W. Gordon¹^,⁹ , Saeid Ghavami¹^,²^,¹⁰

¹Department of Human Anatomy and Cell Science, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, ²Biology of Breathing Theme, Children Hospital Research Institute of Manitoba, University of Manitoba, ³Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, ⁴Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, Center for Comparative Respiratory Biology and Medicine, ⁵Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, ⁶Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, ⁷Centre de biophysique moléculaire - UPR 4301, Centre national de la recherche scientifique (CNRS) CS80054, ⁸LinkoCare Life Sciences AB, ⁹College of Nursing and Children's Hospital Research Institute of Manitoba, Rady Faculty of Health Sciences, University of Manitoba, ¹⁰Health Policy Research Center, Institute of Health, Shiraz University of Medical Sciences

Aquí se describe un protocolo para investigar la Prenilación y guanosina-5'-trifosfato (GTP)-carga de la GTPasa Rho. Este protocolo consta de dos métodos detallados, es decir, ensayo de fraccionamiento de la membrana y un GTPasa-ligado del inmunosorbente. El protocolo puede ser usado para medir la Prenilación y GTP carga de diferentes otras GTPasas pequeñas.