Journal
/
Biología
/
Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation
JoVE Revista
Biología
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología
Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation

Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation

DOI:
10.3791/57649-v

•

06:38 min

•

May 03, 2018

•

, , ,
1Department of Molecular, Cell, and Developmental Biology,University of California, Santa Cruz

Capítulos

  • 00:05Título
  • 01:06Urine Depletion and Bladder Rinsing
  • 02:40Plasmid Delivery
  • 05:16Results: Successful Delivery of pCAG-GFP Plasmid to the Mouse Bladder Urothelium
  • 06:13Conclusion

Summary

Traducción Automática

Traducción Automática

We describe a novel method for the delivery of DNA plasmid into the urothelial cells of mouse bladder in vivo through urethra catheterization and electroporation. It offers a fast and convenient way for generating autochthonous mouse models of bladder diseases.

Tags

Plasmid DNA DeliveryBladder UrotheliumElectroporationGene OverexpressionGenome EditingBladder CancerGene TherapyAnesthesiaCatheterPBS WashingDNA PlasmidTrypan BlueVertical Incision

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit

Transformation of Plasmid DNA into E. coli Using the Heat Shock Method

DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation

Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures

Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development

A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation

A Novel Ex vivo Culture Method for the Embryonic Mouse Heart

In Vivo Microinjection and Electroporation of Mouse Testis

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Read Article