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A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

Article DOI: 10.3791/57725-v • 07:14 min • July 13th, 2018
July 13th, 2018 •
1, 1, 2, 2
1State Key Laboratory of Cardiovascular Disease, Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, 2State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College

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Here, we present a protocol that uses JC-1 dye to assess the mitochondrial membrane potential of cells after being exposed to hypoxia/reoxygenation with or without a protective agent.

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Flow Cytometry-based Assay Measuring Mitochondrial Membrane Potential Cardiac Myocytes Hypoxia/reoxygenation Evaluate Protective Agents Low Material Requirement Radiance Human Cardiac Myocytes Drug Or Vehicle Serum And Glucose-free DMEM Cell Culture Incubator Hypoxic Jar Anerobic Indicator Oxygen Tension Normoxic Environment Reoxygenation Pre-warmed PBS Pre-warmed 25% Trypsin
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