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Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Article DOI: 10.3791/57755-v • 09:18 min • October 20th, 2018
October 20th, 2018 •
1, 1
1Fox Chase Cancer Center

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Here, we show how to use a Proximity Ligation Assay (PLA) to visualize MST1/MST2 heterodimerization in fixed cells with high sensitivity.

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Heterodimerization Protein Isoforms In Situ Proximity Ligation Assay PLA Interactions Proteins Antibodies DNA Oligonucleotides Tissues MST1 MST2 Homodimers Heterodimers Incubation Specific Primer Antibodies Secondary Antibodies DNA Strands Circle-forming DNA Oligonucleotides Replication Amplification Reaction Fluorescence Signal Complimentary Oligonucleotide Probes
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