Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Detecting Protein Subcellular Localization by…
JoVE Journal
Biology

Se requiere una suscripción a JoVE para ver este contenido.

JoVE Journal Biology
Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4',6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans
Counting and Determining the Viability of Cultured Cells
Journal (Biology)
Counting and Determining the Viability of Cultured Cells
Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA
Journal (Biology)
Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA
 

Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4',6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans

Article DOI: 10.3791/57914-v • 09:36 min • July 30th, 2018
July 30th, 2018 •
1, 1, 1
1Department of Science, Borough of Manhattan Community College/CUNY

Capítulos

Resumen
Automatic Translation

This protocol demonstrates how to track a protein nuclear translocation under heat stress by using a green fluorescence protein (GFP) fusion protein as a marker and 4',6-diamidino-2-phenylindole (DAPI) staining. The DAPI staining protocol is fast and preserves the GFP and protein subcellular localization signals.

Tags

Protein Subcellular Localization Green Fluorescence Protein Tagging 4',6-Diamidino-2-phenylindole Staining Caenorhabditis Elegans Cell Biology Nuclear Staining GFP Sub-cellular Localization Changes Extrachromosomal Array Translational Construct Caenorhabditis Genetic Center Radiation Exposure Marker Protein Outcrossing NGM Worm Plates OP50 Clone Liquid LB Broth Bacteria Lawn
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter