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Journal / Bioengineering / 多平行板流室对剪切应力暴露的内皮细胞基因表达分析…
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JoVE Journal Bioengineering
多平行板流室对剪切应力暴露的内皮细胞基因表达分析
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多平行板流室对剪切应力暴露的内皮细胞基因表达分析

Article DOI: 10.3791/58478-v • 08:50 min • October 21st, 2018
October 21st, 2018 •
1,2, 1,2, 2,3, 1,2, 1,2, 1,2,3,4
1Institute of Medical Science, University of Toronto, 2Keenan Research Centre in the Li Ka Shing Knowledge Institute, St. Michael's Hospital, 3Department of Laboratory Medicine and Pathobiology, University of Toronto, 4Department of Medicine, University of Toronto

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本文介绍了流体剪切应力作用下内皮细胞培养和基因表达分析的工作流程。包括一个物理安排, 可同时在受控环境中同时外壳和监测多个流室, 并使用外源参考 RNA 进行定量 PCR。

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生物工程 问题 140 流室 剪切应力 层流 平行板 监测 内皮 基因表达 基因调控 定量 PCR 规范化 荧光素酶 参考 RNA
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