Creation of Colonic Anastomosis in Mice

Cullen K McCarthy; Paul K McGaha; Noah S Rozich; Nathaniel A Yokell; Jason S Lees; William L Berry

doi:10.3791/58742

Creación de Anastomosis de colon en ratones

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JoVE Journal Medicine

Creation of Colonic Anastomosis in Mice

Creación de Anastomosis de colon en ratones

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07:22 min

January 17, 2019

DOI: 10.3791/58742-v

Cullen K McCarthy¹, Paul K McGaha¹, Noah S Rozich¹, Nathaniel A Yokell¹, Jason S Lees¹, William L Berry¹

¹Department of Surgery,University of Oklahoma Health Sciences Center

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Overview

Anastomotic leak after surgery is a significant cause of postoperative complications. This study presents a reliable method for creating a colonic anastomosis to investigate healing mechanisms.

Key Study Components

Area of Science

Surgery
Colonic anastomosis
Postoperative recovery

Background

Anastomotic leaks can lead to severe morbidity and mortality.
Understanding healing mechanisms is crucial for improving surgical outcomes.
The study uses an animal model to simulate human clinical conditions.
Previous methods may not adequately replicate human leak rates.

Purpose of Study

To develop a reproducible method for studying colo-colonic anastomoses.
To investigate the healing mechanisms involved in anastomosis.
To provide insights into reducing anastomotic leak rates.

Methods Used

Securing the limbs of an anesthetized mouse.
Disinfecting the abdomen with chlorhexidine.
Making a midline skin incision to access the abdominal cavity.
Identifying and manipulating the intestines to perform the anastomosis.

Main Results

The method is simple and reproducible.
It mimics human clinical leak rates effectively.
Provides a model for further research on anastomotic healing.
Potentially reduces the risk of postoperative complications.

Conclusions

The developed technique is valuable for studying anastomotic healing.
It may lead to improved surgical practices and outcomes.
Further research can build on this model to enhance patient safety.

Frequently Asked Questions

What is an anastomotic leak?

An anastomotic leak is a complication that occurs when there is a breakdown at the surgical connection between two segments of the intestine.

Why is studying anastomotic healing important?

Understanding anastomotic healing is crucial to reduce complications and improve surgical outcomes in patients undergoing intestinal surgeries.

How does this method compare to previous techniques?

This method offers a more reliable and reproducible approach that closely mimics human clinical conditions.

What animal model is used in this study?

The study uses an anesthetized mouse model to perform the colo-colonic anastomosis.

What are the potential benefits of this research?

The research may lead to reduced anastomotic leak rates and improved patient safety during surgeries.

What surgical technique is highlighted in this study?

The study highlights a technique for performing colo-colonic or colo-rectal anastomosis in an animal model.

Fuga anastomótica o avería después de la cirugía es una causa importante de morbimortalidad postoperatoria. La cirugía para crear una anastomosis colónica es un método confiable y reproducible para el estudio de los mecanismos de cicatrización de las anastomosis.

Este método puede ayudar a responder preguntas clave sobre los mecanismos de curación de las análisismosas colocolones, y puede proporcionar un modelo para reducir las tasas de fugas anastomóticas y sus consecuencias potencialmente devastadoras. Nuestra técnica ofrece un método relativamente simple y reproducible para realizar anastomosis colo colonica o colo-rectal en un modelo animal con tasas de fugas clínicas similares a las pacientes humanos. Comience por asegurar las extremidades de un ratón anestesiado a la superficie quirúrgica, y desinfectar el abdomen expuesto con clorhexidina.

A continuación, coloque una cortina estéril sobre el animal, y haga una incisión en la piel a lo largo de la línea media de la parte inferior del abdomen hasta el xifoide. Utilice fórceps para levantar la línea media de la pared abdominal, y tenga cuidado de no lesionar las estructuras intraabdominales incise la longitud de la fascia abdominal de línea media. Cuando los intestinos estén expuestos, localice el cecum y el intestino delgado, y gire las vísceras del lado izquierdo medialmente para exponer el colon descendente y sigmoide.

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