Activity-based Training on a Treadmill with Spinal Cord Injured Wistar Rats

Jason H. Gumbel; Casey J. Steadman; Robert F. Hoey; James E. Armstrong; Jason D. Fell; Cui Bo Yang; Lynnette R. Montgomery; Charles H. Hubscher

doi:10.3791/58983

Formación basada en una cinta rodante con la médula espinal lesionado ratas Wistar

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

Activity-based Training on a Treadmill with Spinal Cord Injured Wistar Rats

Formación basada en una cinta rodante con la médula espinal lesionado ratas Wistar

Full Text

8,482 Views

06:40 min

January 16, 2019

DOI: 10.3791/58983-v

Jason H. Gumbel¹, Casey J. Steadman¹, Robert F. Hoey¹, James E. Armstrong¹, Jason D. Fell¹, Cui Bo Yang¹, Lynnette R. Montgomery¹, Charles H. Hubscher^1,2

¹Department of Anatomical Sciences and Neurobiology,University of Louisville, ²Kentucky Spinal Cord Injury Rehabilitation Center,University of Louisville

Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

This study provides a protocol for activity-based locomotor treadmill training in rats with spinal cord injury (SCI). It includes quadrupedal and forelimb-only training groups as well as control groups, allowing for assessment of training effects on functional recovery.

Key Study Components

Area of Science

Neuroscience
Rehabilitation
Behavioral Science

Background

Exercise is a key component in rehabilitative therapies for spinal cord injuries.
Activity-based training has shown significant functional benefits in clinical and preclinical studies.
Understanding locomotor training can lead to improved recovery strategies for SCI patients.
This training model allows for in-depth examination of recovery mechanisms.

Purpose of Study

To demonstrate a novel model for assessing locomotor recovery in SCI rats.
To compare outcomes between trained and untrained control groups.
To further the understanding of how locomotor training influences recovery following spinal cord injury.

Methods Used

The main platform is a treadmill designed for rats, allowing for controlled locomotor training.
The study employs a rat model of spinal cord injury, assessing outcomes post-injury through various training types.
Key steps include injury induction via surgical procedures and subsequent rehabilitation through treadmill training.
Monitoring, handling, and care of the rats are essential elements of the protocol.

Main Results

The primary findings will relate to the effects of different training methodologies on recovery outcomes.
Specific changes in locomotor function and behavior will be evaluated across trained groups.
The study aims to clarify the impact of rehabilitation protocols on functional recovery timelines.
Conclusions regarding effective rehabilitation strategies will be drawn based on observed results.

Conclusions

This study establishes a protocol for assessing the effects of treadmill training in SCI rehabilitation.
Implications may extend to understanding the mechanisms behind locomotor recovery.
The findings could inform future therapeutic strategies for rehabilitation in spinal cord injuries.

Frequently Asked Questions

What are the advantages of using a rat model for SCI research?

Rats are commonly used due to their physiological similarities to humans and the feasibility of controlled experiments, allowing researchers to closely monitor recovery and responses to interventions.

How is spinal cord injury induced in the study?

The injury is induced by making a surgical incision and delivering controlled contusions at specified vertebral levels, allowing for standardized injury across subjects.

What types of outcomes can be measured post-training?

Outcomes can include various locomotor functions, behavioral changes, and overall recovery progress, as assessed by comparing trained and control groups.

Can the treadmill training protocol be adapted for different models?

Yes, the protocol can be tailored to different animal models or types of injuries, promoting wider applications in rehabilitative research.

What are the limitations of this study?

Limitations may include the specific injury model used and the controlled settings, which may not fully replicate the complexity of human spinal cord injuries.

Este protocolo muestra nuestro modelo de entrenamiento en cinta deslizante locomotor basado en actividades para las ratas con lesión de la médula espinal (SCI). Incluido, es cuadrúpedos y miembro anterior sólo grupos, además de dos tipos distintos de grupos de control no entrenado. Los investigadores son capaces de evaluar los efectos de entrenamiento en ratas SCI usando este protocolo.

El ejercicio y el entrenamiento locomotor se utilizan comúnmente en terapias de rehabilitación en la población de pacientes lesionados de la médula espinal. La principal ventaja de la formación basada en actividades es que se han documentado muchos beneficios funcionales, tanto en estudios preclínicos en animales, como en la práctica clínica. Demostrando el procedimiento estarán James Armstrong, y Christine Yarberry, ambos técnicos de investigación sénior, y Jason Gumbel, un estudiante graduado de mi laboratorio. Durante la semana anterior a la lesión de la médula espinal, manipule cada rata durante cinco a diez minutos, una vez al día durante cinco días. El día de la lesión, afeitar el cabello de la parte posterior de la primera rata de 200 a 225 gramos, en el lugar de la lesión, y colocar el animal anestesiado en una almohadilla de calentamiento en un ajuste bajo. Administrar lubricante para los ojos, un antibiótico general de acción prolongada, un analgésico y cinco mililitros de solución salina fisiológica. Estimar la ubicación del nivel de lesión objetivo, basado en las protuberancias vertebrales, y utilizar un bisturí número 10 para hacer una incisión de aproximadamente cinco centímetros en el dorso del animal, directamente por encima de las vértebras de la línea media. Usando hemostats, retraer los músculos laterales, usando sutura de seda 4-0. Para las contusiones torácicas medias, utilice rongeurs para eliminar la lámina de vértebras T7 superpuesta, para exponer el nivel T8-T9 de la médula espinal, y utilice un dispositivo de contusión para realizar las contusiones. Después de entregar las lesiones, utilice monofilamentos de 4-0 diámetros para suturar juntos el músculo y la fascia sobre la médula espinal. Y cierra la piel con clips quirúrgicos de heridas de nueve milímetros. Inyectar otros cinco mililitros de solución salina fisiológica. A continuación, coloque los animales en jaulas limpias individuales, en almohadillas de calentamiento con supervisión estrecha durante las primeras 24-48 horas. Uso del credo manual

View the full transcript and gain access to thousands of scientific videos

Explore More Videos

Neurociencia número 143 lesión de la médula espinal formación basada en la actividad entrenamiento locomotor Neurología rehabilitación fisioterapia