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Journal / Biochemistry / A Colorimetric Assay of Citrate Synthase Activity …
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Biochemistry

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A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster

Article DOI: 10.3791/59454-v • 04:30 min • January 16th, 2020
January 16th, 2020 •
*1, *2, 2, 2
1Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 2Department of Anatomy of Physiology, Shanghai Jiao Tong University School of Medicine
* These authors contributed equally

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Resumen
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We present a protocol for a colorimetric assay of citrate synthase activity for quantification of intact mitochondrial mass in Drosophila tissue homogenates.

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Colorimetric Assay Citrate Synthase Activity Drosophila Melanogaster Mitochondrial Function Tissue Homogenate Adult Flies Isolation Of Mitochondria Extraction Buffer HEPES EDTA Triton X-100 Larvae Cultured Cells Mammalian Tissues Carbon Dioxide Anesthesia Fly Thoraxes Homogenization Protein Content Measurement
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