Inducing Apical Periodontitis in Mice

Elisheva Goldman; Eli Reich; Itzhak Abramovitz; Michael Klutstein

doi:10.3791/59521

Inducir la Periodontitis Apical en Ratones

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Inducing Apical Periodontitis in Mice

Inducir la Periodontitis Apical en Ratones

Full Text

13,149 Views

10:26 min

August 6, 2019

DOI: 10.3791/59521-v

Elisheva Goldman^1,2, Eli Reich¹, Itzhak Abramovitz*², Michael Klutstein*¹

¹Institute of Dental Sciences, Faculty of Dental Medicine,Hebrew University of Jerusalem, ²Department of Endodontics,Hadassah Ein Kerem Medical Center

Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

This study presents a novel protocol for locally inducing apical periodontitis in mice, facilitating controlled investigation of periapical inflammation. The method involves drilling the mouse's tooth to expose pulp and offers various analysis techniques, including micro-CT and histology.

Key Study Components

Research Area

Apical periodontitis
Inflammatory response
Dental pathology

Background

Mouse models are advantageous for dental research.
This protocol provides a cost-effective approach.
Procedure visualization enhances technique understanding.

Methods Used

Drilling and pulp exposure technique
Mice as the biological system
Micro-CT and histological analysis

Main Results

Successful induction of apical periodontitis demonstrated.
Significant inflammation observed post-procedure.
Protocols validated for accuracy in results.

Conclusions

The study effectively demonstrates a method for studying dental inflammation in a mouse model.
Findings have implications for understanding periapical diseases.

Frequently Asked Questions

What is apical periodontitis?

Apical periodontitis is an inflammatory condition affecting the dental pulp and surrounding tissues, typically caused by infection.

Why use mice in this study?

Mice provide an accessible and controlled model for studying complex dental diseases.

What are the main advantages of this protocol?

It is cost-effective, technically simple, and allows for controlled observation of periapical inflammation.

What analysis methods are used?

Micro-CT imaging and histology are utilized to assess inflammation and tissue structure.

How is the protocol validated?

Validation is achieved through consistent observations of induced inflammation and tissue responses.

Can this method be applied to other studies?

Yes, it can be adapted for various dental and pulpal research projects.

What long-term effects are monitored post-procedure?

Animal weight and behavior are monitored for 42 days following the procedure.

Aquí, presentamos un protocolo para inducir localmente la periodontitis apical en ratones. Mostramos cómo perforar un agujero en el diente del ratón y exponer su pulpa, con el fin de causar inflamación local. También se demuestran métodos de análisis para investigar la naturaleza de esta inflamación, como la microtomografía y la histología.

Nuestro protocolo permite el estudio de la periodontitis periapical en un modelo de ratón accesible y controlado. Por lo tanto, este protocolo tiene ventajas sobre las muestras de pacientes o modelos in vitro. Las principales ventajas de esta técnica son que el ratón ha sido bien estudiado y que el método es técnicamente simple en términos de requisitos de la instalación y es rentable.

Como este procedimiento es difícil debido a las pequeñas dimensiones de los dientes, es beneficioso visualizar el procedimiento para aprender sobre el posicionamiento y el rendimiento de la técnica. Después de confirmar la falta de respuesta al pellizco del dedo del pie, coloque el ratón en el lado dominante de la mano en una superficie de espuma de poliestireno extruido de celda cerrada y asegure las patas con cinta adhesiva. Usando una sola banda de goma por par de incisivos, y agujas largas ancladas a la superficie de espuma de poliestireno extruido de celda cerrada, apuntalar la boca abierta.

View the full transcript and gain access to thousands of scientific videos