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Bulk Droplet Vitrification for Primary Hepatocyte Preservation

Article DOI: 10.3791/60250-v • 11:07 min • October 25th, 2019
October 25th, 2019 •
1,2,3, 1,2, 1,2, 1,2, 1,2, 3, 1,2, 1,2, 1,2
1Center for Engineering in Medicine, Harvard Medical School, Massachusetts General Hospital, 2Shriners Hospitals for Children, Boston, 3Department of Surgery, University of Amsterdam

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This manuscript describes an ice-free cryopreservation method for large quantities of rat hepatocytes whereby primary cells are pre-incubated with cryoprotective agents at a low concentration and vitrified in large droplets.

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Bulk Droplet Vitrification Primary Hepatocyte Preservation Cryoprotective Agents Rapid Osmotic Dehydration Low CPA Concentration Vitrification Solution BSA University Of Wisconsin Solution Cryopreservation Hepatocytes Transplantation Bio-artificial Liver Devices Cell Types Cryopreservation Blood Products Stem-cells
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