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Mätning av mitokondriell massa och membran potential i hematopoietiska stamceller och T-celler med flödescytometri

Article DOI: 10.3791/60475-v • 07:57 min • December 26th, 2019
December 26th, 2019 •
*1,2, 3, 1,3, 1, 2,4, *1
1Department of Oncology UNIL CHUV, Ludwig Institute for Cancer Research Lausanne, University of Lausanne, 2Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology Lausanne (EPFL), 3Center of Experimental Therapeutics, Department of Oncology, Centre Hospitalier Universitaire Vaudois, 4Hematology Service, Department of Oncology, Centre Hospitalier Universitaire Vaudois (CHUV)
* These authors contributed equally

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Här beskriver vi en tillförlitlig metod för att mäta mitokondriell massa och membran potential i ex vivo odlade hematopoietiska stamceller och T-celler.

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Biologi hematopoietiska stamceller T-celler tumörinfiltrerande lymfocyter mitokondrier mitokondriell membran potential mitokondriell massa metabolism
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