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FLIM-FRET Målinger af protein-protein interaktioner i levende bakterier.

Article DOI: 10.3791/61602-v • 09:26 min • August 25th, 2020
August 25th, 2020 •
1, 2,3, 2,3, 1, 2,3, 1, 1, 2,3, 1, 1,4
1Université de Strasbourg, Laboratoire de Bioimagerie et Pathologies, UMR CNRS 7021, 2Université de Strasbourg, UMR 7242, ESBS, 3CNRS, UMR 7242, ESBS, 4Groupe Méthode Recherche Clinique, Hôpitaux Universitaires de Strasbourg

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Vi beskriver her en protokol til at karakterisere protein-protein interaktioner mellem to meget forskelligt udtrykt proteiner i levende Pseudomonas aeruginosa ved hjælp af FLIM-FRET målinger. Protokollen omfatter bakterier stamme konstruktioner, bakterier immobilisering, billeddannelse og post-imaging dataanalyse rutiner.

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Biologi FRET-FLIM protein-protein interaktioner Pseudomonas aeruginosa bakterier billeddannelse fluorescens
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