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Megakaryocyte Culture in 3D Methylcellulose-Based Hydrogel to Improve Cell Maturation and Study the Impact of Stiffness and Confinement
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Megakaryocyte Culture in 3D Methylcellulose-Based Hydrogel to Improve Cell Maturation and Study the Impact of Stiffness and Confinement

Article DOI: 10.3791/62511-v • 07:53 min • August 26th, 2021
August 26th, 2021 •
1, 1, 1, 1
1Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS

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It is now acknowledged that the three-dimensional environment of cells can play an important role in their behavior, maturation and/or differentiation. This protocol describes a three-dimensional cell culture model designed to study the impact of physical containment and mechanical constraints on megakaryocytes.

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Megakaryocyte Culture 3D Hydrogel Methylcellulose-based Hydrogel Cell Maturation Stiffness Confinement In Vitro Evaluation Bone Marrow Mechanical Aspects Laboratory Practice Sterile Working Conditions Contamination Hydrogel Cell Culture Methylcellulose Luer Lock Syringe Needle Forceps Luer Lock Connector Mouse Lineage Negative Hematopoietic Stem And Progenitor Cells Concentrated Culture Medium
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