Cromatografía líquida acoplada a la detección de índice de refracción o espectrometría de masas para perfiles de metabolitos en sistemas libres de células basados en lisato

Starting, Stopping, and Processing Time Course CFME Reactions for HPLC-RID Quantification

Creating a Sequence Table for Autosampling and Start the HPLC-RID System for Data Acquisition

Extracting and Analyzing Data Post-Run

Starting, Stopping, and Processing Time Course Isotope Tracing CFME Reactions for LC-MS/MS Quantification

Setting Up a Run Sequence and Starting the LC-MS/MS Run

Calculating Negative Mode Masses of 13C-Labeled Glucose-Derived Metabolites and Searching for the m/z Features of These Analytes in Filtered Data

Results: Metabolite Profiling in Lysate-based Cell-free Systems

Conclusion