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Profiling Sensitivity to Targeted Therapies in EGFR-Mutant NSCLC Patient-Derived Organoids

Article DOI: 10.3791/63039-v • 08:52 min • November 22nd, 2021
November 22nd, 2021 •
1,2,3, 1,2, 1,2,3, 1,2,3
1Department of Medicine, University of California, San Francisco, 2Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, 3Department of Cellular and Molecular Pharmacology, University of California, San Francisco

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This protocol describes a standardized evaluation of drug sensitivities to targeted signaling inhibitors in NSCLC patient-derived organoid models.

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Organoids Targeted Therapies EGFR-mutant NSCLC Protocol Details Clear Detailed Protocols Low Biomass Requirements Low Cost Scalability Lung Cancer Treatment Individual Therapies Combination Therapies Matrigel BME2 Handling Pipetting Liquid Handling Organoid Culture Trypsinize Recombinant Enzyme Incubation Centrifuge Tube
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