Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells

Yonatan R. Lewis-Israeli; Brett D. Volmert; Mitchell A. Gabalski; Amanda R. Huang; Aitor Aguirre

doi:10.3791/63097

Generación de organoides del corazón humano autoensamblables derivados de células madre pluripote...

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Biochemistry

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JoVE Journal Biochemistry

Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells

Generación de organoides del corazón humano autoensamblables derivados de células madre pluripotentes

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08:56 min

September 15, 2021

DOI: 10.3791/63097-v

Yonatan R. Lewis-Israeli^1,2, Brett D. Volmert^1,2, Mitchell A. Gabalski^1,2, Amanda R. Huang^1,2, Aitor Aguirre^1,2

¹Institute for Quantitative Health Science and Engineering, Division of Developmental and Stem Cell Biology,Michigan State University, ²Department of Biomedical Engineering, College of Engineering,Michigan State University

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Overview

This protocol allows for the efficient creation of developmentally relevant human heart organoids (hHOs) using human pluripotent stem cells. The method produces complex, functionally relevant heart tissues that can model various cardiovascular diseases.

Key Study Components

Area of Science

Cardiovascular biology
Stem cell research
Organoid technology

Background

Human heart organoids are vital for studying heart development.
They provide insights into congenital heart defects and other cardiovascular diseases.
Current methods lack reproducibility and complexity.
This protocol aims to address these limitations.

Purpose of Study

To develop a reproducible method for creating hHOs.
To model human cardiovascular diseases effectively.
To facilitate high-throughput screening of pharmacological treatments.

Methods Used

Sequential activation of developmental cues.
Three-step differentiation protocol.
Utilization of human pluripotent stem cells.
Creation of a vascular network and heart chambers.

Main Results

Successful generation of all heart cell types.
Creation of a complex 3D structure resembling the developing heart.
Ability to model a range of cardiovascular diseases.
High throughput nature allows for effective drug screening.

Conclusions

This protocol provides a reliable method for hHO creation.
It enhances the understanding of heart development and disease.
The technique is beneficial for future cardiovascular research.

Frequently Asked Questions

What are human heart organoids?

Human heart organoids are 3D structures created from stem cells that mimic the developing human heart.

How does this protocol improve upon previous methods?

This protocol is more reproducible and allows for the creation of complex heart tissues.

What diseases can be modeled using hHOs?

HOs can model various cardiovascular diseases, including congenital heart defects.

What is the significance of using pluripotent stem cells?

Pluripotent stem cells can differentiate into any cell type, making them ideal for creating heart tissues.

Can this method be used for drug screening?

Yes, the high throughput nature of the method allows for effective pharmacological screening.

Is this protocol cost-effective?

Yes, the protocol is designed to be simple and cost-effective.

Aquí, describimos un protocolo para crear organoides cardíacos humanos (hHO) relevantes para el desarrollo de manera eficiente utilizando células madre pluripotentes humanas por autoorganización. El protocolo se basa en la activación secuencial de señales de desarrollo y produce tejidos cardíacos humanos altamente complejos y funcionalmente relevantes.

Este protocolo nos permite crear una estructura 3D compleja que se asemeja mucho al corazón humano en desarrollo de una manera simple y rentable, que es reproducible a través de líneas celulares. Consiste en un único protocolo con tres pasos de diferenciación, que da lugar a todos los tipos celulares del corazón, incluyendo cámaras y una red vascular. Esta técnica nos permite modelar una amplia gama de enfermedades cardiovasculares humanas y detectar tratamientos farmacológicos efectivos.

Gracias a la naturaleza de alto rendimiento de su diseño. Este método es útil para investigar el corazón humano en desarrollo, así como las enfermedades comúnmente asociadas con él. Uno de ellos son los defectos cardíacos congénitos.

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