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Biochemistry

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JoVE Journal Biochemistry
An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
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An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

Article DOI: 10.3791/63665-v • 07:45 min • June 6th, 2022
June 6th, 2022 •
1, 1, 1, 2, 2, 1, 2,3, 1,3
1Department of Pathology, University of New Mexico School of Medicine, 2Department of Physics and Astronomy, University of New Mexico, 3Comprehensive Cancer Center, University of New Mexico Health Sciences Center

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The present protocol describes sample preparation and data analysis to quantify protein phosphorylation using an improved single-molecule pull-down (SiMPull) assay.

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SiMPull Optimized Single-molecule Pull-down Assay Quantification Protein Phosphorylation Antibody Labeling Fixation Conditions Autofluorescence Reduction Single Molecule Analysis Algorithms Phosphorylation Status Novel Information Western Blotting Mass Spec Analysis Researchers Demonstration Julian Rojo Piranha Etching Cover Glass Coating Array Drawing And Sample Imaging
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