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Production of High-Titer Recombinant Newcastle Disease Virus from Allantoic Fluid

Article DOI: 10.3791/63817-v • 07:24 min • May 25th, 2022
May 25th, 2022 •
1, 1, 1, 1, 1, 1, 1
1Department of Pathobiology, University of Guelph

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Here we provide a detailed procedure for production, purification, and quantification of high-titer recombinant Newcastle disease virus. This protocol consistently yields > 6 × 109 plaque-forming units/mL, providing virus quantities appropriate for in vivo animal studies. Additional quality control assays to ensure safety in vivo are described.

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Recherché Newcastle Disease Virus Allantoic Fluid Tangential Flow Filtration Centrifugation Processes Time Management Pause Steps Research Assistant Purification PBS Depth Filter
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