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Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Article DOI: 10.3791/64200-v • 09:49 min • November 18th, 2022
November 18th, 2022 •
1, 2, 2, 2,3, 1
1Department of Chemistry, University of Florida, 2Division of Protective Immunity, Children’s Hospital of Philadelphia, 3Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania

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A novel sample preparation method is demonstrated for the analysis of agar-based, bacterial macrocolonies via matrix-assisted laser desorption/ionization imaging mass spectrometry.

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Microbial Cooperation Imaging Mass Spectrometry Bacterial Colonies Agar Tissue Infection Spatial Metabolism Imaging Mass Spectrometry Workflow Bacterial Co-cultures MALDI Matrix Application Metabolites Microbial Pathogens Sample Types Cellular Biochemistry Tissue Biochemistry Dehydration Bacterial Culture Macrocolonies Agarose Bacterial Colonies Microscope Slides Dry Box Dessicant Agar Surface Deformations
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