Determinación de la permeabilidad intestinal usando amarillo de lucifer en un modelo enteroide apical-out

Overview

This protocol describes a method using lucifer yellow in an apical-out enteroid model to assess intestinal permeability. It is particularly relevant for studying inflammatory bowel diseases like necrotizing enterocolitis.

Key Study Components

Area of Science

• Neuroscience
• Biology
• Gastroenterology

Background

• Lucifer yellow is a fluorescent dye used to evaluate permeability.
• Enteroids are 3D models that mimic intestinal tissue.
• Paracellular permeability is crucial for understanding intestinal health.
• This method can be applied to various intestinal diseases.

Purpose of Study

• To develop a reliable method for assessing intestinal permeability.
• To utilize enteroid models for studying human intestinal diseases.
• To investigate the mechanisms underlying necrotizing enterocolitis.

Methods Used

• Application of lucifer yellow to the media of apical-out enteroids.
• Growing enteroids embedded in BMM for 7-10 days.
• Using 50% LWRN conditioned media for enteroid generation.
• Conducting quantitative and qualitative analysis of permeability pathways.

Main Results

• Demonstrated a straightforward technique for permeability assessment.
• Showed potential applications in various intestinal diseases.
• Highlighted the role of enteroids in studying disease mechanisms.
• Involved collaboration among research scholars and assistants.

Conclusions

• The lucifer yellow method is effective for studying intestinal permeability.
• Apical-out enteroids provide a valuable model for research.
• This technique can enhance understanding of intestinal diseases.

Frequently Asked Questions