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Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
JoVE Revista
Biología del desarrollo
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JoVE Revista Biología del desarrollo
Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
DOI:

07:17 min

December 16, 2022

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Capítulos

  • 00:05Introduction
  • 00:59Embryo Collection and Processing
  • 03:12Electroporation
  • 04:29Embryo Culture and Genotyping
  • 05:43Results: Genome Editing by CRISPR RNP Electroporation of Zygotes
  • 06:41Conclusion

Summary

Traducción Automática

Here, we describe a simple technique intended for the efficient generation of genetically modified mice called CRISPR RNP Electroporation of Zygotes (CRISPR-EZ). This method delivers editing reagents by electroporation into embryos at an efficiency approaching 100%. This protocol is effective for point mutations, small genomic insertions, and deletions in mammalian embryos.

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