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Extrahepatic Bile Duct and Gall Bladder Dissection in Nine-Day-Old Mouse Neonates

Article DOI: 10.3791/64424-v • 06:10 min • August 23rd, 2022
August 23rd, 2022 •
1,2,3, 1,2,3, 1,2, 1,2, 1,2, 1,2
1Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, 2Research Laboratory for Pediatric Surgery, 3Research Animal Facility, University Medical Center Hamburg-Eppendorf

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For the observation of murine neonatal bile duct disorders, an intact bile duct and efficient preparation are required. Therefore, a new approach for isolating the entire extrahepatic bile duct system in murine neonates was successfully developed while maintaining the integrity of the bile duct.

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Extrahepatic Bile Duct Gall Bladder Dissection Mouse Neonates Extrahepatic Biliary System Technique Contaminating Cells Atraumatic Removal Dissection Protocol Murine Neonatal Bile Duct Disorders Duodenal Atresia Orientation Partially-digested Milk Training Step-by-step Approach Instruments Operation Table Peritoneum Decapitation Skin Removal Atraumatic Forceps Microscope
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