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Mitokondri ve Lizozom için Floresan Boyalarla Mitopajinin Görselleştirilmesi
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Mitokondri ve Lizozom için Floresan Boyalarla Mitopajinin Görselleştirilmesi

Article DOI: 10.3791/64647-v • 07:56 min • November 30th, 2022
November 30th, 2022 •
1,2, 1, 3, 1, 1, 1,2
1Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, 2Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, 3Department of Pharmacy, Shanghai East Hospital, Tongji University

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Mitofaji, mitokondriyal kalite kontrolünün birincil mekanizmasıdır. Bununla birlikte, in vivo mitofajinin değerlendirilmesi, güvenilir kantitatif tahlillerin eksikliği nedeniyle engellenmektedir. Burada, hücre pervasıtalı yeşil-floresan mitokondri boyası ve kırmızı-floresan lizozom boyası kullanılarak canlı hücrelerde mitofajinin gözlemlenmesi için bir protokol sunulmaktadır.

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