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Generating Genetically Modified Plasmodium berghei Sporozoites
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Generating Genetically Modified Plasmodium berghei Sporozoites
DOI:

10:16 min

May 05, 2023

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Capítulos

  • 00:04Introduction
  • 00:57Generation of Schizonts in Culture
  • 02:25Transfection of Plasmodium Schizonts
  • 04:58Selection of the Transfected Parasites
  • 05:52Infection of Mosquitoes with the Transgenic Lines
  • 07:15Collection of Sporozoites
  • 08:53Results: Detecting the Lysis of Plasmodium and its Parasitophorous Vacuole in Host Hepatocytes
  • 09:42Conclusion

Summary

Traducción Automática

Malaria is transmitted through inoculation of the sporozoite stage of Plasmodium by infected mosquitoes. Transgenic Plasmodium has allowed us to understand the biology of malaria better and has contributed directly to malaria vaccine development efforts. Here, we describe a streamlined methodology to generate transgenic Plasmodium berghei sporozoites.

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