An Efficient and Fast Method for Labeling and Analyzing Mouse Glomeruli

Lin Bai; Yaping Wu; Wenshu Dai; Qiuxiao Shi; Lei Wu; Jie Zhang; Lily Zheng

doi:10.3791/65973

Un método eficiente y rápido para etiquetar y analizar glomérulos de ratón

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Biology

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An Efficient and Fast Method for Labeling and Analyzing Mouse Glomeruli

Un método eficiente y rápido para etiquetar y analizar glomérulos de ratón

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09:50 min

February 9, 2024

DOI: 10.3791/65973-v

Lin Bai^1,2, Yaping Wu², Wenshu Dai¹, Qiuxiao Shi², Lei Wu², Jie Zhang^1,2, Lily Zheng³

¹Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital,Sichuan University, ²Core Facility of West China Hospital,Sichuan University, ³Regenerative Medicine Research Center, West China Hospital,Sichuan University

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Overview

This study presents a groundbreaking method for labeling and analyzing glomeruli in CUBIC-cleared mouse kidneys, aiming to simplify the analysis of kidney structures. Utilizing fluorescein isothiocyanate (FITC)-Dextran and various microscopy techniques, researchers can effortlessly quantify glomerulus number and volume across the entire kidney.

Key Study Components

Research Area

Kidney structure analysis
Glomeruli labeling techniques
Light sheet and confocal microscopy

Background

Traditional glomeruli analysis methods are often complex and time-consuming.
Simplifying the process can facilitate significant research breakthroughs.
Current methodologies may require specialized equipment that is not universally available.

Methods Used

Fluorescein isothiocyanate (FITC)-Dextran labeling
Mouse kidneys as the biological model
Light sheet fluorescence microscopy (LSFM) and confocal microscopy

Main Results

A reliable and quick method for glomeruli analysis was established.
Quantitative data on glomeruli number, volume, and distribution can be obtained.
The methodology offers potential applications in kidney disease research.

Conclusions

The study demonstrates a rapid and intuitive approach for glomeruli analysis.
It holds significant relevance for advancing kidney biology research.

Frequently Asked Questions

What is the significance of analyzing glomeruli?

Analyzing glomeruli is crucial for understanding kidney function and disease mechanisms.

Can this method be used in other types of tissues?

While this method is tailored for kidney tissues, adaptations may allow its use in other transparent tissues.

What microscopic techniques are utilized in this study?

Light sheet fluorescence microscopy and confocal microscopy are employed for detailed imaging.

Is specialized equipment required for this method?

No, this method is designed to be accessible and does not necessitate specialized equipment.

How does this method improve upon traditional glomeruli analysis?

It simplifies the labeling and counting process, making it faster and more intuitive.

What types of data can be obtained from this analysis?

Researchers can obtain data on glomeruli quantity, volume, and distribution.

What future research directions does this study suggest?

Further exploration into labeling biotargets in transparent tissues is planned to meet evolving research needs.

Este estudio presenta un conjunto de métodos fáciles de usar, completos y simples para etiquetar y analizar glomérulos de riñones de ratón aclarados por CUBIC. Los datos como el número y el volumen del glomérulo se pueden obtener de forma fácil y fiable utilizando isotiocianato de fluoresceína (FITC)-Dextrano, microscopía de fluorescencia de lámina de luz (LSFM) o microscopía confocal común y software como Imaris.

El etiquetado y el recuento de glomérulos con métodos convencionales puede ser complicado, tedioso, lento y se requiere un instrumento voluminoso. Nuestro método ofrece un método de etiquetado instantáneo e intuitivamente preciso para el análisis de los glomérulos dentro de todo el riñón. Nuestra técnica es un método rápido, fácil, preciso y barato para analizar los glomérulos en todo el riñón.

No requiere ningún equipo especial, por lo que se puede llevar a cabo en cualquier laboratorio. Se trata de un método innovador, rápido y sencillo para etiquetar glomérulos, que permite una observación intuitiva de todos los glomérulos de todo el riñón. Tiene un gran potencial para ayudar a los investigadores a realizar avances significativos en el estudio del riñón al proporcionar información como la cantidad, el volumen, la distribución de los glomérulos y más.

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