Portable Paper-Based Immunoassay Combined with Smartphone Application for Colorimetric and Quantitative Detection of Dengue NS1 Antigen

Muhammad Hatta Prabowo; Thanit Chalermwatanachai; Werasak Surareungchai; Patsamon Rijiravanich

doi:10.3791/66130

Inmunoensayo portátil en papel combinado con una aplicación para teléfonos inteligentes para la d...

JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection

Portable Paper-Based Immunoassay Combined with Smartphone Application for Colorimetric and Quantitative Detection of Dengue NS1 Antigen

Inmunoensayo portátil en papel combinado con una aplicación para teléfonos inteligentes para la detección colorimétrica y cuantitativa del antígeno NS1 del dengue

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06:00 min

January 26, 2024

DOI: 10.3791/66130-v

Muhammad Hatta Prabowo^1,2, Thanit Chalermwatanachai³, Werasak Surareungchai², Patsamon Rijiravanich^4,5

¹Department of Pharmacy, Faculty of Mathematics and Natural Sciences,Universitas Islam Indonesia, ²School of Bioresources and Technology,King Mongkut's University of Technology Thonburi, ³Department of Otolaryngology,Phramongkutklao Hospital and College of Medicine, ⁴Sensor Technology Laboratory, Pilot Plant Development and Training Institute,King Mongkut's University of Technology Thonburi, ⁵Biosciences and Systems Biology Research Team, National Center for Genetic Engineering and Biotechnology,National Sciences and Technology Development Agency at King Mongkut's University of Technology Thonburi

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Overview

This study presents a smartphone app-integrated Dengue NS1 Paper-based Analytical Device (DEN-NS1-PAD) designed for quantifying Dengue NS1 antigen concentration in clinical serum samples. The device aims to enhance dengue management by improving clinical decision-making, particularly in resource-limited healthcare settings.

Key Study Components

Area of Science

Neuroscience
Biotechnology
Diagnostic Devices

Background

Dengue fever is a significant global health concern.
Current diagnostic methods may be inadequate in resource-limited settings.
NS1 antigen is a critical biomarker for dengue detection.
There is a need for simple, cost-effective diagnostic solutions.

Purpose of Study

To develop a sensitive and specific device for dengue detection.
To integrate a smartphone application to enhance measurement accuracy.
To provide scalable solutions for dengue diagnostics.

Methods Used

Development of a paper-based analytical device (PAD).
Fabrication of microfluidic methods based on PEC.
Integration of a smartphone application for enhanced sensitivity.
Testing the device with real serum samples.

Main Results

The DEN-NS1-PAD demonstrated improved sensitivity in detecting NS1.
The smartphone application significantly enhanced measurement efficiency.
Results showed better performance compared to traditional observation methods.
The device is suitable for use in various healthcare settings.

Conclusions

The DEN-NS1-PAD is a promising tool for dengue diagnostics.
Integration with mobile technology offers significant advantages.
This innovation can improve clinical decision-making in dengue management.

Frequently Asked Questions

What is the DEN-NS1-PAD?

The DEN-NS1-PAD is a smartphone app-integrated device for detecting Dengue NS1 antigen in blood samples.

How does the smartphone application enhance the device?

The application improves sensitivity and measurement accuracy of NS1 detection.

What are the advantages of using this device?

It is simple, cost-effective, and suitable for resource-limited healthcare settings.

What biomarker does the device detect?

The device specifically detects the Dengue NS1 antigen.

How does the device compare to traditional methods?

It offers improved sensitivity and efficiency over traditional observation methods.

Para abordar las necesidades urgentes de diagnóstico del dengue, aquí presentamos un dispositivo analítico en papel basado en el dengue NS1 integrado en la aplicación para teléfonos inteligentes (DEN-NS1-PAD) para cuantificar la concentración del antígeno NS1 del dengue en muestras clínicas de suero/sangre. Esta innovación mejora el manejo del dengue al ayudar a la toma de decisiones clínicas en diversos entornos de atención médica, incluso en los de recursos limitados.

El objetivo de la investigación es la detección del dengue mediante un biomarcador específico NS1, con un dispositivo analítico basado en papel. Nuestra investigación se centra en el desarrollo de un dispositivo sencillo, barato, sensible y específico para detectar el dengue. También desarrollamos una aplicación para teléfonos inteligentes para mejorar la sensibilidad y la medición de NS1 en muestras reales.

Nuestra sensibilidad está desarrollando un método para fabricar un método microfluídico basado en nuestro análisis de conos llamado PEC. El uso de la fórmula está en nuestro equipo, donde algunas técnicas de alta calidad, tienen como objetivo encarnar la comercialización de varios diseños de PEC, ofreciendo soluciones escalables para más aplicaciones de suministro. Nuestra investigación combina PEC con una aplicación para teléfonos inteligentes, lo cual es muy prometedor para la medición del dengue NS1.

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